FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_exp269-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_exp269-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9786245
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1557751.5917749862179007Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1429541.4607645731330046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA61500.053.3238819
ACACGAG63550.051.4382720
GAGCACA190950.051.13829416
AGAGCAC196450.050.90051315
GATCGAG46950.049.5743068
AGATAGA184250.049.2567337
GCACACG103300.048.28100218
CACGAGA69700.047.50143421
GATAGAT191550.047.3247348
AGCACAC126050.047.17503717
AAGAGCA310350.045.2790914
CAGATAG73500.042.7623756
GCACAAG79800.042.63095518
ATAGATC219900.040.9845669
CACGTAG38100.040.5099821
GAGCAAG89750.039.46460316
ATCGAGA60850.038.249829
AGCACAA89850.038.0573217
GCAGATC103200.037.5792775
ACGAGAT88550.037.38980522
Produced by FastQC (version 0.11.2)