FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds983-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds983-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12160069
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1873221.5404682325404568No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC482670.39693031347108304Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG16100.023.4955481
CAGATCG89250.023.4114656
CGGGGAC13750.023.1806951
TAGATCG101550.022.781596
GAGCACA46750.022.60868516
AGAGCAC47500.022.54689615
GATCGAG26350.021.3852798
CGGGGAG13650.020.7845251
GATAGAT87050.020.6663598
ATAGATC87800.020.6094999
GCACACG19450.020.33351118
AGATAGA94400.019.2795737
CGCCGTA235000.019.07792947
CGGGGAT21950.018.9889161
GGTCGCC241500.018.8111244
TCGCCGT239250.018.6950746
GTCGCCG242850.018.63426245
CCGTATC233400.018.60883349
GCCGTAT246300.018.4867748
GTGGTCG252000.017.95810342
Produced by FastQC (version 0.11.2)