FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds983-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds983-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448353
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2558761.770970019904691No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC631110.43680411185967005Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG102950.024.478136
TAGATCG124650.023.6143446
CGGGGAG18700.022.6621781
CGGGGTT24850.021.8455621
CGGGGAC16550.021.3737771
CGCCGTA284200.020.07282847
GCACACG26300.019.69504718
GAGCACA54400.019.68652716
TCGCCGT289650.019.61069946
GGTCGCC296800.019.57484244
GGGGGTA39550.019.4820231
CGGGGTG18350.019.468031
GCCGTAT298900.019.40171648
GTCGCCG296950.019.37624445
CCGTATC286000.019.2735949
AGAGCAC56100.019.15321415
AGATCGG443450.018.6586177
GTGGTCG311050.018.63293342
GATCGGA444100.018.584028
ATAGATC103350.018.4910589
Produced by FastQC (version 0.11.2)