FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds983-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds983-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296907
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1410300.8153480850651507No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC690060.3989499394313677Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA72850.030.1222116
AGAGCAC75000.029.81945815
GCACACG30050.027.60293218
GATCGAG45150.025.4271438
GATAGAT148100.024.3896838
AAAGGGG135600.023.97838666
AGATAGA152400.023.7935267
ATAGATC152700.023.7234489
AAGGGGG139150.023.13987567
CACACGA29250.021.17867519
AGCACAC50950.021.01974117
GGGGGAC23150.020.57821
TAGATCG200100.020.2733446
ACACGAG31250.020.0477520
CAGATCG179100.019.7971616
AGGGGGG173650.019.22781868
CGGGGAC14700.019.0630381
AAGAGCA201650.018.88386314
AGATCGA66050.018.4942467
CAGATAG71250.018.4710676
Produced by FastQC (version 0.11.2)