FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12519010
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2330561.861616853089821No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC408740.32649546569576987Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC13450.025.5206851
TAGATCG99450.023.8960136
CAGATCG83900.023.1521596
CGGGGAG15750.022.2386281
GATCGGA340550.019.4755868
AGATCGG342850.019.3655837
CGGGGAT25200.018.9028321
ATAGATC81700.018.3777479
TCGGAAG350350.018.27118910
ATCGGAA359950.018.2312369
CGGGGTT23150.018.1559411
AGAGCAC44250.018.11238915
GGGGGTA30350.017.887991
CGGGGTG19500.017.6027281
GAGGGTA39700.017.4688051
CGCCGTA222000.017.31002647
GGGGGAT27650.017.2278981
GAGCACA45050.017.16882516
CGGAAGA373250.017.1031111
GATAGAT88650.017.0950898
Produced by FastQC (version 0.11.2)