FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9040878
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1120761.2396583606149756No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC444750.49193231011412825Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18150.033.3596118
AGAGCAC45450.032.7266615
GAGCACA44450.032.281716
GATCGAG20850.030.3842168
AGATAGA80800.026.2936237
GATAGAT80150.026.0703168
AGCACAC29100.025.61729817
CACACGA16000.025.59291619
ATAGATC84300.024.8280129
TAGATCG117650.023.7403166
GGGGACC8650.023.4743272
CAGATCG101300.023.4258736
ACACGAG17200.023.400420
GGGGGAC14050.022.933391
AAGAGCA105750.022.10778214
CGGGGAC9800.021.4428221
CAGATAG36800.020.2585036
CACGAGA21700.019.83795421
AAAGGGG80550.019.72766
AGATCGA34050.019.3247497
Produced by FastQC (version 0.11.2)