FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12671932
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2191851.72968889037599No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC530900.4189574249609294Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG103450.027.641826
TAGATCG128100.026.1752876
CGGGGAC13950.025.1101341
CGGCGCT20300.024.3302361
CGGGGTG16850.024.3225561
GCACACG22550.024.21220218
AGAGCAC54750.023.46091815
GATCGAG25600.022.6954128
GAGCACA55400.022.55344816
GATAGAT98300.022.289018
ATAGATC100700.022.2790579
AGATAGA99950.022.0964057
CGGGGAT21850.021.9630361
CGGGGAG17250.021.7279071
CGGGGTT22050.021.1283861
AGATCGG467100.020.05927
GATCGGA469400.019.9308518
GGGGGAC20750.019.7510871
GGGGGAT29350.019.4537241
CACACGA22200.019.23363519
Produced by FastQC (version 0.11.2)