FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds774-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds774-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685158
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2503462.8824576363492755No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1046051.2044110193504827Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA123650.052.730316
AGAGCAC128100.052.0742215
ACACGAG40950.051.87806720
CACACGA42800.049.308319
GCACACG61000.048.99700518
CACGAGA45500.046.9976721
AGCACAC72250.046.88986617
GTGACTG46050.044.950771
GCACAAG64900.044.7045118
AAGAGCA216850.043.89989514
TGACTGG50100.041.998942
GACTGGA51300.040.738593
GAGCAAG64000.039.81006616
AGCACAA75150.038.51394717
CACGTAG23350.035.3729821
CACAAGA82850.034.85041419
ACGAGAT63350.033.81067322
CAGATCG183700.033.6288536
AGAGATC106600.033.3234817
AGAGAGA109200.033.0113715
Produced by FastQC (version 0.11.2)