FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds395-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds395-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518942
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3274783.8441158538231623No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC710700.8342585264696014Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC100550.051.06196215
GAGCACA97800.050.88679516
GCACACG50650.048.8525118
ACACGAG34950.047.26522420
CACACGA36600.045.7078919
AGCACAC61950.044.12214717
CACGAGA40100.041.36979721
AAGAGCA170350.040.14551514
GCACAAG53250.039.3689818
GTGACTG43550.037.958311
TGACTGG47700.035.303392
GACTGGA49100.034.720113
ACGAGAT52050.032.5442422
CACGTAG23450.032.08817721
AGCACAA66400.031.67767717
CAGATCG125800.031.1325176
CGAGATC57350.030.39102723
CACAAGA72650.029.04865819
CCGTATC219900.028.72858249
ACTGGAG60650.028.6252174
Produced by FastQC (version 0.11.2)