FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds1002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds1002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98811.89358372456964No Hit
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA480.577825929938606Illumina Single End PCR Primer 1 (100% over 25bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC450.5417118093174431Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGG420.5055976886962802Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA150.18057060310581438Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTAGATCGGAAGAGCGTCGTGTAGG140.1685325628987601Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.13241844227759722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG252.5097688E-670.4121252
TAACTGA207.257911E-570.4121252
GTGACTG207.257911E-570.4121251
ACACGTA207.481167E-569.9879520
CACACGT207.481167E-569.9879519
CTTTAGC207.481167E-569.9879517
ACTGAAG207.481167E-569.987954
AACTGAA207.481167E-569.987953
CACGTAG207.481167E-569.9879521
AGCTAAA207.481167E-569.9879521
TAGCTAA207.481167E-569.9879520
GATAAAA150.00214914969.9879530
ACGTAGA252.6029247E-669.9879522
GTTCAGA207.481167E-569.9879510
CTGAAGA207.481167E-569.987955
TGCTCTA150.00214914969.9879521
AGACGTG252.6029247E-669.9879514
AAGATCA252.6029247E-669.987958
GCTAAAA150.00214914969.9879522
CTGGAGT207.481167E-569.987955
Produced by FastQC (version 0.11.2)