FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_exp269-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_exp269-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344573
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG651680.6299728369648511TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG382530.3697881004851529TruSeq Adapter, Index 5 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG245800.028.40642770
CGTGTGC86850.027.9084871
GCTTGAA288650.027.4993860
CTTGAAA310300.026.75385561
TGCTTGA279600.026.23647559
ACGTGTG48650.026.2070751
CTGCTTG275800.025.88695758
GCGTGAA58600.024.4273960
GTGTGCT100200.024.3244342
TGAAAAA449300.023.91406263
TCTGCTT329800.022.65647557
GAAAAAA491000.022.4319364
CTTCTGC343100.021.79861555
TGCGTGA51600.021.70459659
CTGCGTG49700.021.54815758
CGTGAAA70400.021.52626861
TTGAAAA406800.021.44829662
TTCTGCT323000.021.06390456
CCGTCTT380150.021.05494751
GCCGTCT359850.021.02699550
Produced by FastQC (version 0.11.2)