FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_exp269-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_exp269-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9190862
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG446060.4853298852708266TruSeq Adapter, Index 3 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC59000.028.2563881
GTGTGCT71100.023.6384962
ACGTGTG33250.023.2788931
AAAAAGG164650.019.4925770
GCTTGAA187350.019.03596560
TGTGCTC95750.018.3988591
CTTGAAA204150.018.22387361
CTGCTTG174850.018.17461458
TGCTTGA180750.017.98827459
GTGCTCT100500.017.6988832
TGAAAAA287950.016.55461763
GAAAAAA297450.016.41418664
GCGTGAA35550.016.24421560
GCCGTCT231000.015.12057250
TCTGCTT218300.015.10226657
CTGCGTG30400.015.08143458
TGCGTGA31850.015.05439159
CCGTCTT243750.014.97577751
TCCGATC191600.014.7475255
TCTTCCG202400.014.7220142
Produced by FastQC (version 0.11.2)