FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_exp269-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_exp269-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9786245
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1748951.787151251578108TruSeq Adapter, Index 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG255150.034.33381370
CGTGTGC133500.032.7176131
ACGTGTG75500.032.0573461
TGAAAAA497750.030.54419363
CTTGAAA486950.030.35212361
TTGAAAA503900.029.59492962
GAAAAAA521600.029.41596664
GTGTGCT157100.027.9516052
ATGCCGG22050.025.87234570
GCTTGAA566500.025.81860
GACGTGT39750.025.730141
AAAAAAG367100.025.22680569
TGCTTGA589700.024.3868259
CTGCTTG621000.023.01641358
TCTGCTT676400.021.31754157
CTTCTGC703550.020.62423155
TTCTGCT696400.020.57968156
TGTGCTC218400.020.1535933
AGGGGGG11000.020.0606171
GTGCTCT228050.019.3467544
Produced by FastQC (version 0.11.2)