FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds983-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds983-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12160069
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG562630.4626865192952441TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG234750.019.69451758
GCTTGAA240150.019.57244560
TGCTTGA240100.019.43075659
GCCGTCT254600.018.25509850
TGCCGTC260000.018.01056549
CCGTCTT263200.017.75169851
TATGCCG269750.017.35957747
ATGCCGT273000.017.20419748
GTATGCC278500.016.9649746
GATCTCG261300.016.74236940
CTTGAAA287000.016.73107761
CGATCAG284150.016.6894334
AGTCACG287200.016.6096829
GTCACGA288400.016.5648430
CTTCTGC288050.016.463455
CACGATC289800.016.46073232
CGTATGC290200.016.3172445
CGTGTGC25850.016.2592721
CTCGTAT277500.016.25684443
TCTGCTT287850.016.23166757
Produced by FastQC (version 0.11.2)