FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds983-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds983-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448353
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG258060.17860859296557885TruSeq Adapter, Index 8 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC127350.017.47848349
TATGCCG144300.017.38995247
GTATGCC156650.017.22540946
GTCACAC360200.016.77037230
GCCGTCT109900.016.59149750
ATGCCGT135500.016.45302248
GCACACG390600.016.27167312
ACACGTC394200.016.20297814
AGTCACA378200.016.09250529
CACACGT396700.016.0919913
CAGTCAC379350.016.08062428
ACGTCTG400650.015.95965516
GAACTCC392050.015.91678922
CACGTCT402400.015.85545815
CCAGTCA384550.015.81767127
TCCAGTC386250.015.8024226
CGTATGC158250.015.79065345
AGCACAC405700.015.66610111
AGATCGG412000.015.6420061
GCGTGAA53750.015.62742360
Produced by FastQC (version 0.11.2)