FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds983-a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds983-a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296907
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG785430.4540869647966541TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC42050.021.6573261
GCTTGAA321100.018.76932760
TGCTTGA320500.018.32392559
CTGCTTG325000.017.9514458
CTTGAAA375150.016.52230861
GCCGTCT405300.015.04227750
CCGTCTT415350.014.82155151
TGCCGTC422450.014.67194849
GTGTGCT62950.014.6300882
TCTGCTT410250.014.40027857
TGAAAAA466450.014.38377463
TCCGATC148050.014.3329655
ATGCCGT437100.014.23624648
TGTGCTC82450.014.231531
AAAAAGG254650.014.12892370
TATGCCG438600.014.12372347
GAAAAAA484400.013.973556564
GTGCTCT86650.013.8211582
CTTCTGC444500.013.76307455
TTGAAAA470650.013.70512962
Produced by FastQC (version 0.11.2)