FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12519010
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG248830.19876172317140092TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT128450.015.93936256
CATCTCG166050.015.23860240
TGCCGTC148100.015.1478955
TATGCCG160250.014.93838953
GTATGCC168100.014.63648546
GCACACG297850.014.58203512
CTGCTTG130700.014.54024264
TAGATCG76900.014.5257666
ATGCCGT152950.014.50737948
CACACGT302100.014.40006313
ACACGTC301600.014.37751814
ACGTCTG305150.014.25613316
CCGTCTT149150.014.21996557
AGTCACC288850.014.13994335
CACGTCT309650.014.09416315
CAGTCAC293050.014.04471928
CGTATGC171500.014.0401545
AGCACAC310800.013.98582311
CGTCTGA312950.013.87833417
GAACTCC305850.013.85727722
Produced by FastQC (version 0.11.2)