FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9040878
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG225580.24951116473422164TruSeq Adapter, Index 10 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC32100.019.9688151
CTGCTTG94300.016.40433958
ACGTGTG20950.016.3850671
GCTTGAA117600.015.98141260
TGCTTGA104400.015.923659
GTGTGCT39750.015.8575412
AAAAAGG136550.015.40399570
TGCCGTC115800.015.14179549
TATGCCG130600.014.95337547
ATGCCGT119500.014.73154348
GTATGCC144900.014.49201146
GCCGTCT101900.014.45959350
GCGTGAA32500.014.43010860
CCGTCTT118200.014.15325151
TCCGATC89450.014.01493555
CTGCGTG29550.013.85723558
TGCCGGC33750.013.79196249
TATCTCG161750.013.78283440
TGTGCTC51150.013.6958991
CGTATGC145050.013.584245
Produced by FastQC (version 0.11.2)