FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12671932
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG623540.4920638778680315TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC28900.018.6638451
CTGCTTG267250.016.94627264
GCTTGAA285050.016.73499560
TGCTTGA279650.016.55753959
GCCGTCT306350.015.68549556
CCGTCTT313850.015.3663657
TGCCGTC322400.015.14344955
TATGCCG330400.014.96744753
ATGCCGT330250.014.86827154
CTTGAAA333550.014.69004161
TAGATCG94400.014.4664196
CTTCTGC328200.014.41763561
GTATGCC347450.014.3135552
TATCTCG349550.014.13739246
GTCACGC366100.014.09105130
TCTGCTT328600.014.05886557
CGTATGC351500.014.04900651
TCACGCC368350.013.99547631
CGTCTTC347600.013.95492158
AGTCACG374250.013.8964129
Produced by FastQC (version 0.11.2)