FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds774-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds774-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685158
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG947081.0904579974250324TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC82500.033.795411
GCTTGAA214800.031.67527860
CTGCTTG196350.031.62067458
ACGTGTG40900.031.6010631
TGCTTGA204350.031.20557259
GTGTGCT93850.029.6636032
AAAAAGG190100.029.60515870
CTTGAAA249300.027.85337461
CTTCTGC243400.026.65861555
TGAAAAA319450.026.53549663
TCTGCTT242800.026.34986957
TGCGTGA26350.026.16640559
CCGTCTT271500.025.8456851
CGTCTTC268150.025.32021352
GTCTTCT256250.025.14408553
TCTTCTG260350.024.80188654
GCGTGAA34650.024.44390360
GCCGTCT277300.024.38390250
TTGAAAA296800.024.20937262
CTGCGTG27650.024.04954758
Produced by FastQC (version 0.11.2)