FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds395-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds395-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518942
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG525750.6171541019999901TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG120600.032.7057758
TGCTTGA131850.030.76494859
GCTTGAA144950.029.96445860
CGTGTGC75150.029.7788411
ACGTGTG43550.029.5934471
CCGTCTT154000.028.15794251
GCCGTCT149750.027.60154550
TGCCGTC167500.027.22575449
GTGTGCT84850.026.4513422
TCTGCTT157800.026.08243257
ATGCCGT184200.025.28941348
CGTCTTC164050.025.15289152
CTTCTGC162150.025.14544155
GCGTGAA28550.025.00788360
TATGCCG203700.024.01963847
CTTGAAA189750.023.6645261
GTCTTCT166750.023.52827553
AAAAAGG171950.022.89843470
TGCGTGA25450.022.8282859
CTGCGTG23550.022.73779158
Produced by FastQC (version 0.11.2)