FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds1002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds1002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1131.360298543397135TruSeq Adapter, Index 14 (97% over 38bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC610.7343204526303118TruSeq Adapter, Index 14 (96% over 30bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA490.5898639701456603TruSeq Adapter, Index 14 (96% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400.4815216082821716No Hit
AGAGCACACGTCTGAACTCCAGTCACAGTTCAGACGTGTGCTCTTCCGAT230.27687492476224873TruSeq Adapter, Index 14 (96% over 33bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT190.22872276393403151TruSeq Adapter, Index 14 (96% over 29bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA190.22872276393403151TruSeq Adapter, Index 14 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGTTCAGACGTGTGCTCTTCCG100.1203804020705429TruSeq Adapter, Index 14 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTTCAGACGTGTGCTCTTCCGA100.1203804020705429TruSeq Adapter, Index 14 (97% over 34bp)
GAGCACACGTCTGAACTCCAGTCACAGTTCAGACGTGTGCTCTTCCGATC100.1203804020705429TruSeq Adapter, Index 14 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGCA150.002147682270.043
TCTGCAC150.002147682270.044
TTTGGTG150.002147682270.019
CTGCACT150.002147682270.045
CGTCTGC150.002147682270.042
GATCTTT252.2549686E-456.00000415
CCATTGC200.006712555452.56
ACGTGTG800.048.1251
ATGTCAA604.0017767E-946.66666460
CGATCTT451.4912657E-646.66666414
GTCAATC551.1146949E-744.54545662
TGTCAAT551.1146949E-744.54545661
CATGTCA658.123607E-943.07692359
TCAATCT503.080797E-642.00000463
CACATGT701.5637852E-840.057
ACATGTC701.5637852E-840.058
CGATCTA350.001185133840.013
GAGATCG458.436743E-538.88888525
AATCTCG555.9322374E-638.1818265
CAATCTC555.9322374E-638.1818264
Produced by FastQC (version 0.11.2)