FastQCFastQC Report
Fri 24 Sep 2021
H3YFLDMXY_n02_cDNA_C2_210910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YFLDMXY_n02_cDNA_C2_210910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197792147
Sequences flagged as poor quality0
Sequence length104
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84775304.286080174861543Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14281160.7220286657791323Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8323010.42079577608306157Clontech SMART CDS Primer II A (100% over 25bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4632430.23420697283800654Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3535860.1787664502170554Clontech SMART CDS Primer II A (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790630.14108901907010496Clontech SMART CDS Primer II A (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2532290.1280278331778258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)