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        Note that additional data was saved in multiqc_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-09-27, 16:09 based on data in: /beegfs/mk5636/logs/html/H3WNHAFXY/merged


        General Statistics

        Showing 36/36 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H3WNHAFXY_n01_3_79T0_0
        73.1%
        45%
        10.6
        H3WNHAFXY_n01_3_79T0_1
        72.7%
        45%
        9.0
        H3WNHAFXY_n01_3_79T0_2
        67.9%
        45%
        6.8
        H3WNHAFXY_n01_3_spo0A_0
        71.9%
        45%
        8.5
        H3WNHAFXY_n01_3_spo0A_1
        75.3%
        46%
        9.2
        H3WNHAFXY_n01_3_spo0A_2
        68.9%
        45%
        7.5
        H3WNHAFXY_n01_3_spoVG_0
        70.5%
        45%
        8.0
        H3WNHAFXY_n01_3_spoVG_1
        70.9%
        45%
        8.0
        H3WNHAFXY_n01_3_spoVG_2
        67.7%
        45%
        5.2
        H3WNHAFXY_n01_WT_2
        74.5%
        44%
        9.3
        H3WNHAFXY_n01_WT_3
        73.2%
        44%
        8.0
        H3WNHAFXY_n01_WT_4
        73.8%
        44%
        9.1
        H3WNHAFXY_n01_WT_5
        73.6%
        44%
        8.6
        H3WNHAFXY_n01_undetermined
        68.8%
        45%
        34.6
        H3WNHAFXY_n01_ydcC_2
        74.2%
        45%
        8.6
        H3WNHAFXY_n01_ydcC_3
        74.1%
        45%
        8.8
        H3WNHAFXY_n01_ydcC_4
        73.9%
        44%
        8.4
        H3WNHAFXY_n01_ydcC_5
        71.8%
        44%
        7.7
        H3WNHAFXY_n02_3_79T0_0
        71.8%
        45%
        10.6
        H3WNHAFXY_n02_3_79T0_1
        71.3%
        45%
        9.0
        H3WNHAFXY_n02_3_79T0_2
        66.0%
        45%
        6.8
        H3WNHAFXY_n02_3_spo0A_0
        70.1%
        45%
        8.5
        H3WNHAFXY_n02_3_spo0A_1
        73.6%
        46%
        9.2
        H3WNHAFXY_n02_3_spo0A_2
        67.4%
        45%
        7.5
        H3WNHAFXY_n02_3_spoVG_0
        68.4%
        45%
        8.0
        H3WNHAFXY_n02_3_spoVG_1
        68.8%
        45%
        8.0
        H3WNHAFXY_n02_3_spoVG_2
        66.0%
        46%
        5.2
        H3WNHAFXY_n02_WT_2
        73.0%
        45%
        9.3
        H3WNHAFXY_n02_WT_3
        71.1%
        45%
        8.0
        H3WNHAFXY_n02_WT_4
        72.5%
        45%
        9.1
        H3WNHAFXY_n02_WT_5
        72.7%
        44%
        8.6
        H3WNHAFXY_n02_undetermined
        76.9%
        44%
        34.6
        H3WNHAFXY_n02_ydcC_2
        72.3%
        45%
        8.6
        H3WNHAFXY_n02_ydcC_3
        70.9%
        45%
        8.8
        H3WNHAFXY_n02_ydcC_4
        71.7%
        45%
        8.4
        H3WNHAFXY_n02_ydcC_5
        70.7%
        44%
        7.7

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 18/18 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        34,584,088
        19.7
        WT_2
        9,253,367
        5.3
        WT_3
        7,962,771
        4.5
        WT_4
        9,055,547
        5.2
        WT_5
        8,616,035
        4.9
        ydcC_2
        8,582,675
        4.9
        ydcC_3
        8,751,358
        5.0
        ydcC_4
        8,445,452
        4.8
        ydcC_5
        7,685,077
        4.4
        3_79T0_0
        10,625,274
        6.0
        3_spo0A_0
        8,452,542
        4.8
        3_spoVG_0
        8,017,067
        4.6
        3_79T0_1
        8,975,476
        5.1
        3_spo0A_1
        9,197,425
        5.2
        3_spoVG_1
        7,994,541
        4.6
        3_79T0_2
        6,778,114
        3.9
        3_spo0A_2
        7,511,579
        4.3
        3_spoVG_2
        5,158,291
        2.9

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        194,296,296
        175,646,679
        19.7
        2.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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