FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_ydcC_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_ydcC_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685077
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG85130.11077312563036129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT11150.025.4302167
GTGGAGA23000.023.6033041
CTAGTTG11550.022.7294048
CTCTAGT11750.022.047066
CACCTAC9950.020.7528832
CGGTGGG25450.019.9429328
CTAACCT10200.019.90201442
GTGATAG28850.019.6613674
TAGCCTC29400.019.2875238
ACCTACT11650.019.22651933
GTTACCT14050.018.93194214
GGCGTGA29450.018.9095331
ACCTTAG9900.018.7386449
ACGGTGG28300.018.430617
TACTATC10500.018.33278736
ACCTCTA14150.018.3022717
CTACTAT10850.017.74152235
GACACCT14600.017.7388530
TGTACTC16300.017.6163522
TGGAGAC31950.016.8787232
Produced by FastQC (version 0.11.5)