FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_ydcC_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_ydcC_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8445452
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG90070.10664911718164996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT15300.026.9986537
CTAGTTG15500.026.8747028
CTCTAGT15350.026.6836016
ACCTTAG12950.023.245379
ACCTACT13650.020.5115433
CACCTAC13500.020.48020232
TGTACTC20650.020.0109582
TGTTACC20700.019.61311513
ACCTCTA20850.019.1349317
CTAACCT14800.018.68325842
GTTACCT21250.018.61115314
CCTTAGA16600.018.34451110
GTACCAC9200.018.2671935
TACTATC15400.018.1813336
TACCTCT22400.017.96720316
GTGATAG30600.017.9661833
TTACCTC22350.017.53774315
GACACCT21750.017.37781330
TGGACAC21450.017.29475428
ACTATCT15900.017.17036637
Produced by FastQC (version 0.11.5)