FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_ydcC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_ydcC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8751358
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184220.2105044725630011No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG93030.1063035016965367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT14900.027.0172317
CTAGTTG16900.024.6473188
CTCTAGT17100.022.9286526
ACCTTAG16400.021.129589
ACCTACT13000.019.65268533
ACCTCTA19350.018.99134317
CTAACCT13900.018.63412342
GTGATAG31850.018.5805243
CTACTAT15200.018.42080735
TGTTACC19650.018.34623113
TGTACTC21500.018.2425332
ACCTAGA16950.018.1700255
TACCTCT20450.018.14094516
CCTTAGA18950.017.91655710
GACACCT20950.017.54073130
CCTAGAT16850.017.44694156
TACTATC15450.017.44313436
TGGACAC21050.017.29143728
TCTAACC15600.016.82775741
CCTACTA16250.016.58373334
Produced by FastQC (version 0.11.5)