FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_ydcC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_ydcC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8582675
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG105300.12268902177934035No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG95100.11080461511125611No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA90150.10503718246350933No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC87210.10161167701212034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT12150.020.7447267
CTAGTTG11800.019.5791198
GTGATAG39350.018.6878873
CACCTAC9250.017.02652732
GGCGTGA38200.016.9614281
TAGCCTC41800.016.9171267
CGGGGAG13300.016.5898671
GCGGGGT13100.016.5757981
CGTGATA47900.015.7918022
GATAGCC48850.015.7681415
ACCTACT10150.015.17179233
AGGTGGT11650.015.0249596
TGATAGC49000.014.8639824
CTCTAGT15350.014.8242996
GCGTGAT53050.014.7882451
GTTACCT14950.014.51453814
ACCTTAG8700.014.4841059
TACTATC11650.014.42068236
TGTTACC14400.014.33993913
CTAACCT10800.014.25958842
Produced by FastQC (version 0.11.5)