FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34584088
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2856603182.59876912179959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT3400.024.7353866
TCTAGTT3600.021.4126287
ACTCTAG5350.017.686885
CTAGTTG4250.017.3111258
ATAGGGT2852.6921043E-1017.19179549
GTAGGGA12100.016.18769618
CTCGGGT1858.912434E-515.1612541
TAGGGTG3251.7298589E-915.07537550
TCGTGTA16800.015.00716914
GGTGTAG4800.014.57987353
TAGATCG6300.014.44551232
GATAGGG3403.261448E-914.41282948
TGTACTC5650.014.2705722
TGTTACC5500.014.00775213
AGCGTCG17750.013.6154610
TGTAGGG14750.013.51289517
TAGGGAA15000.013.05829419
GTTACCT5203.6379788E-1212.79457414
GTGTAGA8900.012.58636429
GAGTGTA10400.012.45368227
Produced by FastQC (version 0.11.5)