FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_WT_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_WT_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8616035
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA90100.10457246285559425No Hit
GAAAACTGCAACGACAACCGCTTCACATGCATGCCCATTTTGTTCAGGGA86380.10025493164779391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGCG12950.016.2194217
GTACCAC17750.015.9774925
GGTACCA18700.015.5412584
CACCTAG4100.015.3713015
GGGGAGG16000.015.3206862
ACCACCC15700.015.1622317
GTGATAG20600.015.1284763
GGGGGAT18050.014.9412461
GGGAGGT14800.014.9056623
CTAGTTG12050.014.5248498
TAGCCTC20250.014.5213887
CGGGGAG21000.014.5101951
TACCACC18100.014.5062186
GATTCGA43300.014.3131454
TCTAGTT10600.014.2009187
GGGGAAT26900.014.0619251
CCAGGGG33700.013.91732242
TGCTACG32800.013.87147170
GCCAGGG34000.013.79444141
CTCTAGT9500.013.6348286
Produced by FastQC (version 0.11.5)