FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_WT_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_WT_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9055547
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT17300.027.5185767
CTCTAGT18750.023.897846
CTAGTTG19250.023.6395158
ACCTTAG17300.022.8635649
TGTACTC22950.020.2925422
CACCTAC15200.020.03180332
CCTTAGA20350.019.436310
TACCTCT23450.019.40085616
GTTACCT23150.018.89829414
CGGGGAG15450.018.8135151
ACCTCTA23900.018.7426117
ACCTACT16100.018.6945333
TTACCTC25200.018.05367515
GTGATAG31200.017.9554773
TGTTACC24650.017.74849313
TGGACAC24900.017.14756628
CTAACCT17800.017.10751242
GACACCT25750.016.85326630
TACTATC18500.016.64847836
CTACTAT18350.016.21227835
Produced by FastQC (version 0.11.5)