FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_WT_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_WT_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7962771
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG85800.1077514347706345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT13300.024.2153537
CTAGTTG14900.022.5536988
CTCTAGT15050.020.9353266
ACCTACT13300.018.15683733
TGTACTC18350.018.1298832
ACCTCTA17450.018.04961817
CACCTAC13600.017.24196632
GTGATAG29400.016.1981833
GTTACCT19250.016.1811814
GGCGTGA27650.015.9606691
TGTTACC19400.015.87576313
TACCTCT20350.015.82138116
ACCTTAG14000.015.5015329
GTACCAC15250.014.9232575
TACTATC16050.014.82835736
CTAACCT15900.014.74942142
CTACTAT16450.014.68064235
CCTTAGA15500.014.67822510
TGGACAC19800.014.67067928
ACTATCT16500.014.6368937
Produced by FastQC (version 0.11.5)