FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_WT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_WT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9253367
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG112530.12160978809118886No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA93620.10117398348082379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG16300.019.9838851
GTGATAG38950.019.3300673
AGGTGGT12200.019.2259656
TCTAGTT12550.017.5728347
GGTAGCG13350.017.5686577
CTAGTTG13050.016.630498
TAGCCTC44000.016.6279477
CGTGATA48150.016.2198242
GGCGTGA38500.016.102641
GGTGGTG15100.015.9962117
GCGTGAT53750.015.8347931
GATAGCC49900.015.7875185
TGATAGC50800.015.3022894
GGGAGGT15600.015.2646453
TCTCGAG14950.015.2168856
CCAGGGG31800.014.41837842
GCCAGGG31600.014.39887341
GAGGTAG17800.014.1626455
GGTGCCC15850.014.13247513
GTGGGGC14450.014.04873212
Produced by FastQC (version 0.11.5)