FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_spoVG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_spoVG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158291
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53660.10402670186695556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT14350.034.4009326
ACTCTAG15800.031.6889065
GTACTCT17000.029.0435053
TCTAGTT17500.029.0076087
TGTACTC18950.026.2414262
TACTCTA21750.025.7578754
CTAGTTG20050.024.967938
TAGTTGT21650.023.1207079
GTTACCT21950.022.00435314
TTGTACT23400.021.5537261
TGTTACC22750.021.23077813
TTACCTC23550.020.80438215
CACCTTA20200.020.4499158
ACCTCTA23650.020.27268617
ACCTTAG20000.019.9525249
ACCTACT17050.019.91145333
ACACCTA17000.019.76356531
CACCTAC16700.019.69984232
CTCTAAT24500.019.4263219
CCTTAGA21550.018.6787710
Produced by FastQC (version 0.11.5)