FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_spoVG_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_spoVG_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7994541
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130730.16352408474732946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG20200.037.96135
CTCTAGT23850.032.7326056
TACTCTA25250.031.0641464
CTAGTTG25050.030.323228
TCTAGTT25650.030.1605387
GTACTCT27900.028.2400023
TACCTCT27850.027.51974716
ACCTCTA30200.025.49419217
TTACCTC31950.024.2073315
TGGACAC31550.023.5165128
ACCTTAG21450.023.4981739
TAGTTGT35900.022.7174919
GACACCT33750.022.39878730
CTAACCT22900.022.16245342
TGTTACC35850.021.28250313
ACCTACT24850.021.26605433
GTTACCT36600.021.22875814
ACACCTA25100.021.05463631
TCTAACC24000.021.00096541
CACCTAC26650.020.48643932
Produced by FastQC (version 0.11.5)