FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_spoVG_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_spoVG_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8017067
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128640.16045768358927273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG18950.034.179935
TACTCTA22400.030.1677074
CTCTAGT23050.028.5533876
TCTAGTT25000.027.0252087
GTACTCT25400.026.3303763
CTAGTTG24900.025.3049768
TACCTCT28000.023.24795216
ACCTCTA28750.022.3980317
ACCTACT22900.020.02057333
ACACCTA23300.019.97728231
TTACCTC33100.019.56045515
TAGTTGT33950.019.1772779
TGGACAC33150.019.10901828
GACACCT34050.018.70660230
CTAACCT24200.018.51388442
TGTTACC34450.018.48997713
ACCTTAG22300.018.3651629
GTTACCT34250.018.18920114
CCTACTA26200.017.89963334
TCTAACC25350.017.81208241
Produced by FastQC (version 0.11.5)