FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_spo0A_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_spo0A_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7511579
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79970.10646230306570695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG9850.028.0789975
CTCTAGT12500.022.4043886
CTAGTTG15650.018.5648238
TCTAGTT16750.017.7638447
CGGGGAG12150.017.5834221
AGGTAGC13350.017.306766
GGTAGCG11600.017.2008447
ACCTCTA16400.015.57827517
GTACTCT18800.014.5277983
CTAACCT13100.014.16043942
TACCTCT19300.013.9628416
TCTAACC13400.013.84350741
TACTCTA22750.013.6972694
CACCTAG3951.869921E-913.2948935
GAGTCGA16450.013.1962714
AGGACTA13800.013.1877453
TGGGAGT16000.013.1335171
ACACCTA15200.013.12407631
TAGTTGT24450.013.0279229
TTACCTC19900.013.01441915
Produced by FastQC (version 0.11.5)