FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_spo0A_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_spo0A_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197425
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99600.10829117932464793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT16850.031.373266
ACTCTAG18350.029.0034185
TCTAGTT20050.026.7130957
GTACTCT20950.025.2413483
CTAGTTG23400.022.7383218
TACTCTA29100.021.6599084
TAGTTGT26200.020.574149
TGTACTC25600.020.1107252
TACTATC15250.019.5079336
TGTTACC26400.019.22322313
CTAACCT16050.018.53617942
CTTAGAG20450.018.48476211
CACCTAC16550.018.18627432
CCTTAGA21150.017.87297210
ACCTTAG21650.017.7843119
ACCTCTA28900.017.3166917
TGGTAGT11350.016.95873815
ACCTACT17550.016.55176233
ACACCTA18300.016.4468831
CCTACTA18450.016.31343334
Produced by FastQC (version 0.11.5)