FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_spo0A_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_spo0A_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8452542
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90220.10673712121158346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG14950.040.048695
TACTCTA19600.031.6218054
CTCTAGT20700.027.9052126
GTACTCT23300.025.2501473
TCTAGTT24500.024.8616077
CTAGTTG27400.022.6124788
TAGTTGT27350.022.3963859
ACCTCTA25300.021.9941417
TACCTCT25900.021.75512916
ACACCTA18650.020.8299131
CACCTAC19300.020.67251832
TTGTACT28950.020.5674441
TGTACTC29350.020.5237452
CTAACCT19650.019.94962142
GGACACC29100.019.12261829
GACTATC19850.019.04162255
TACTATC20350.018.91884636
ACCTACT20800.018.84518833
AGGACTA20100.018.63055653
ACCTTAG19850.018.3387629
Produced by FastQC (version 0.11.5)