FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_79T0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_79T0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778114
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94180.13894720566812538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT10050.024.7319986
CTAGTTG11050.022.808948
ACTCTAG11300.022.6181055
TCTAGTT12100.021.4087287
ACCTCTA15300.016.92646417
GTTACCT15900.016.2886914
TGTTACC16850.015.16274213
TACTATC10650.015.11724336
TACTCTA21150.014.8995384
TCTAACC10850.014.516541541
CTAACCT11250.014.3115242
TTACCTC18550.014.14977915
TAGGGTT13200.014.05135622
CTCTAAT18900.013.88764519
TACCTCT17900.013.88133516
CACCTAC11450.013.75473432
TAGTTGT20700.013.6970539
GTACTCT20500.013.1526373
CGGGGAT21900.013.1136211
TGGACAC18950.012.92779328
Produced by FastQC (version 0.11.5)