FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_79T0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_79T0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8975476
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91950.10244582014368932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG12400.026.5419045
CTCTAGT13900.023.4231686
TCTAGTT14700.022.8609927
CTAGTTG14250.022.3536458
TACTATC11050.017.10371436
GTTACCT19600.016.60679414
TCTAACC12800.016.40654641
TGTTACC20600.015.97080613
ACCTCTA20800.015.4798417
CGGGGAG14050.015.206681
CTAACCT13650.014.87222842
TACTCTA25800.014.6574714
GTACTCT23800.014.5658233
TAGTTGT27100.014.465939
TGGACAC23250.014.45042228
TAGGGTT12450.014.05436422
GACTAGT1500.00592247414.0060284
GACACCT25000.013.99923830
ACACCTA16000.013.99923731
TGTACTC24850.013.9515322
Produced by FastQC (version 0.11.5)