FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n02_3_79T0_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n02_3_79T0_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10625274
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG20750.033.9147265
TACTCTA24300.030.2588214
CTCTAGT24450.028.922096
TCTAGTT27850.026.269377
GTACTCT27350.026.1182793
CTAGTTG30300.023.6823888
TACCTCT31150.022.2451616
ACCTCTA34050.020.35037217
TGTACTC36800.019.5074392
TTACCTC36850.019.2794715
ACCTTAG19000.018.4222329
CTAACCT25500.018.11778342
TAGTTGT40850.017.9938099
TACTATC25600.017.91012436
TCTAACC25750.017.67011841
TGGACAC38100.017.26941328
TGTTACC40250.017.21727813
ACCTACT27700.017.05684733
CCTACTA27700.016.80423234
GTTACCT41450.016.80326814
Produced by FastQC (version 0.11.5)