FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_ydcC_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_ydcC_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685077
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT113450.14762376486273332TruSeq Adapter, Index 7 (97% over 38bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG84190.10954997588182916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG25650.038.47928
ACTCCAG27050.036.74625823
CACACGT31050.032.23787712
CTCCAGT32150.030.80828324
GCACACG32550.030.5372111
AGCACAC34750.029.00679610
CCAGTCA34000.028.8231326
TCACGAG35600.028.70746630
CACGAGA34900.028.4809831
CTCGTAT32450.027.93489844
CATCTCG32150.027.3246641
CAGTCAC37950.027.1142527
TCGTATG34200.026.91483145
CACGTCT37550.026.65759514
CGTATGC35400.026.20020346
GTCACGA38450.025.851429
ACACGTC42000.024.58315313
ACGTCTG41400.024.09418315
ACGAGAT42000.023.6663432
TCCAGTC44000.022.90877325
Produced by FastQC (version 0.11.5)