FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_ydcC_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_ydcC_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8445452
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG94370.11174061494873216No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT93420.11061574916298145TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG22200.034.5265823
TATCTCG24450.028.62948641
CACACGT28850.027.29600712
AGTCACC28500.027.26280628
CCAGTCA30500.025.4750826
GCACACG31600.024.80980511
CTCCAGT31600.024.03449824
AGCACAC34550.022.8940610
CAGTCAC34650.022.82798827
TCGTATG33400.022.2152845
TCTAGTT12350.021.8215797
CTCGTAT32650.021.54642944
CTCATTA36050.021.35894836
CGTATGC35400.020.86131346
CACGTCT38000.020.8155214
CGCTCAT37250.020.67087434
ACCGCTC38600.020.21994432
CACCGCT39150.019.93588631
ACACGTC43900.019.21387513
ACGTCTG40950.019.05958215
Produced by FastQC (version 0.11.5)