FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_ydcC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_ydcC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8751358
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG100950.11535352570423928No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG91020.10400671530064248TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG25750.048.2516343
ACTCCAG38800.043.8397323
AGTCACT40550.042.2066928
ATCGGGG35250.041.5028642
CACACGT43450.039.3894512
CACTCCG43550.039.29900731
ACTCCGG44800.038.51498432
CCAGTCA45300.037.5492626
GCACACG46200.036.96908611
GTCACTC46100.036.74559429
CTCCAGT48550.034.81939724
TCACTCC49350.034.53843330
CAGTCAC49700.034.36582627
AGCACAC51400.033.63757710
CGGGGGT17000.032.11711544
CTCCGGA55500.030.77426533
CACGTCT55950.030.65203714
CGGGGGG22250.030.35904744
ACACGTC58550.030.00821513
ACGTCTG58250.029.74216715
Produced by FastQC (version 0.11.5)