FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_ydcC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_ydcC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8582675
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG112890.1315324185058854No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT105220.12259581074664949TruSeq Adapter, Index 27 (97% over 39bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG99920.11642057983088022No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC91580.1067033296728584No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA89010.10370892524766463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA24050.037.5462636
ACTCGAT23900.035.87816637
ACTCCAG25200.035.55506523
CACACGT25300.035.55286812
GCACACG27650.032.40461711
ACATTAC28650.032.12870432
AGTCACA28000.031.99956128
CATTACT29400.031.78527833
CCAGTCA31350.028.69179226
CAGTCAC33600.027.39545627
ATTACTC34650.026.666334
CTCCAGT35550.025.89275424
TCGTATG35850.025.09031145
CGTATGC35350.024.85114546
AGCACAC38200.023.73004210
CTCGTAT35050.023.26644144
GTCACAT39550.022.65455629
TTACTCG40650.022.38591835
CACATTA43400.021.6932531
ACACGTC44750.021.58629413
Produced by FastQC (version 0.11.5)