FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34584088
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT176700.036.9400412
GCACACG184200.035.32171611
ACTCCAG183450.035.0631623
CTCCAGT183150.034.87248624
CCAGTCA182650.034.1480526
TCGGGGG49150.031.76725843
CAGTCAC195900.031.74924727
AGCACAC209200.031.56885110
CACGTCT209850.030.89055814
ACACGTC215300.030.56246613
GAACTCC214050.030.37431721
ACGTCTG222500.029.20495615
TCCAGTC229400.028.01296425
TGAACTC235000.028.00934620
AACTCCA236050.027.82922422
TACTCGA59500.026.11952236
CGTCTGA263500.024.96745116
AGAGCAC263850.024.8973188
CTGAACT266550.024.64260719
ACTCGAT64650.023.66393537
Produced by FastQC (version 0.11.5)