FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_WT_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_WT_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8616035
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA94640.10984170793178069No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC90450.10497868218966148No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA88100.10225120951806718No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA87360.10139234578318217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG17450.019.85647223
GGGGGGA10150.017.2433555
AGTCACG21000.016.83311328
GTACCAC16250.016.8019225
CACCTAG4400.016.7064595
CTCCAGT21950.016.2640224
TGGGAGT39050.016.139881
GGTACCA17050.015.8082624
GTGATAG19100.015.7610593
TACCACC16200.015.5573366
GAGTCGA42800.015.5391624
ACCACCC14300.015.4194677
TAGCCTC18850.015.0396777
ACCCACG43700.014.89674211
AGCACAC25700.014.57170810
CGGTGGG18700.014.4177661
GGGAGTC45900.014.4139172
CCCACGT46500.014.07500112
GGAGTCG47600.014.0458083
GGGGAGG11000.014.002092
Produced by FastQC (version 0.11.5)