FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_WT_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_WT_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9055547
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT149760.1653792973522196TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG31300.037.4594223
TATCTCG31450.035.05504241
CACACGT34450.034.44062812
GCACACG35400.033.41750311
AGTCACC36050.032.62079628
AGCACAC41400.029.50432210
CTCCAGT39750.029.40829724
CCAGTCA41100.029.0384326
TCGTATG40250.028.43429245
CAGTCAC44950.027.4077627
CGTATGC41500.026.90315246
CTCGTAT40850.026.73148544
CACCGCT44150.026.6360131
CTCATTA45050.026.25925836
ACCGCTC46500.025.66622532
CGCTCAT46350.025.52275734
CACGTCT48100.024.66693514
ACGTCTG49850.023.94141615
ACACGTC52050.023.6691413
CCGCTCA50000.023.51959433
Produced by FastQC (version 0.11.5)