FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_WT_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_WT_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7962771
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG168920.2121372070099718TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT133720.16793149018099351TruSeq Adapter, Index 6 (97% over 36bp)
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG89100.11189572072335122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG39750.057.58398443
ACTCCAG59100.051.93655823
ATCGGGG52000.051.893442
ACTCCGG60450.051.41356732
CACTCCG60750.050.81431
GTCACTC61900.049.64378729
CACACGT63750.048.9168612
AGTCACT64050.048.30523328
GCACACG67150.046.4921811
CTCCAGT66350.046.47250424
CCAGTCA66350.046.26150526
TCACTCC67200.045.98884630
CAGTCAC70900.043.83568227
CTCCGGA71900.043.12865433
AGCACAC72850.043.04666510
TCCGGAG74900.041.35447734
CACGTCT76050.041.18934614
TCCAGTC75950.040.6445125
CGGGGGT23800.039.852344
ACACGTC80950.039.3878813
Produced by FastQC (version 0.11.5)